Risperidone and 9-Hydroxyrisperidone on Oasis MCX 

Condition:

500 μL MeOH

Equilibrate:

500 μL H₂O

Load:

500 μL (250 μL human plasma, diluted 1:1 with 4% H₃PO₄ in H₂O)

Wash 1:

500 μL 2% FA

Wash 2:

500 μL MeOH

Elute:

250 μL (125 μL x 2) 5% NH₄OH in MeOH

Options:

1. Dilute 250 μL H₂O with 2% FA

2. Evaporate/ Reconstitute

3. Direct inject

Inject:

10 μL

Column:

ACQUITY UPLC BEH C₁₈ 2.1 x 50 mm, 1.7 μm

Mobile Phase A:

0.1% HCOOH in H₂O

Mobile Phase B:

0.1% HCOOH in MeOH

Flow Rate:

0.3 mL /min

Injection Volume:

10.0 μ L

Column Temperature:

40 °C

Sample Temperature:

10 °C

Instrument:

ACQUITY UPLC with Quattro Premier

ESI+

Capillary:

3.5 kV

Source Temp.:

120 °C

Desolvation Temp.:

350 °C

Cone Gas Flow:

0 L /Hr

Desolvation Gas Flow:

700 L /Hr

Collision Cell Pressure:

2.59 e^-3 mbar