• Application Note

Clonazepam

Clonazepam
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of clonazepam using Symmetry C18 columns.

Introduction

Clonazepam has been analyzed in this application brief.

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 250 mm, 5 μm (p/n: WAT054275)

Mobile phase:

Water/methanol/acetonitrile 40:30:30

Flow rate:

1.5 mL/min

Injection volume:

25 μL of sample with 40 μg/mL of clonazepam and 80 μL/mL of o-dichlorobenzene (internal standard)

Detection:

UV @ 254 nm

USP Tailing Factors

1. 1.08
2. 1.04 

Results and Discussion

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WA31763.60, June 2003

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