• Application Note

Warfarin in Rat Plasma

Warfarin in Rat Plasma
  • Waters Corporation
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Platelet-rich plasma vials in rack

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of warfarin in ratplasma using XTerra MS C18 columns.

Introduction

Warfarin in rat plasma has been analyzed in this application brief.

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.5% HCOOH

Mobile phase B:

ACN

Flow rate:

0.2 mL/min

Isocratic mobile phase composition:

40% A; 60% B

Injection volume:

20 μL

Detection:

MS ESI-

Instrument:

Alliance 2790, Micromass Quattro Ultima

MS Conditions

Ion source:

ESI-

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 30 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying ga flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

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WA20738.118, June 2002

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