• Application Note

Dibucaine - LC/UV

Dibucaine - LC/UV
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of dibucaine using XTerra RP18 columns.

Introduction

Dibucaine has been studied in this application brief.

Experimental

HPLC Method

Column:

XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)

Mobile phase:

At pH 3.0: H2O/ACN/100 Mm NH4COOH, pH 3.0, 30:60:10

At pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0, 35:55:10

At pH 10.0: H2O/ACN/100 mM NH4HCO3, pH 10.0, 20:70:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 280 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile phase pH

USP Trailing

3

1.23

7

1.19

10

1.18

 

Results and Discussion

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WA20738.034, June 2002

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