• Application Note

Analysis of Catecholamines Using Atlantis T3

Analysis of Catecholamines Using Atlantis T3
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights analysis of catecholamines

Introduction

Compounds analysed in this application brief :

  1. Norepinephrine (NE)
  2. Epinephrine (E)
  3. 3,4-Dihydroxyphenylalanine (DOPA)
  4. Dopamine (DA)
  5. 3-Methoxy-4-hydroxyphenylglycol (MHPG)
  6. 4-Hydroxy-3-methoxymandelic acid (VMA)
  7. Serotonin(5-HT)
  8. 3,4-Dihydroxyphenylacetic acid (DOPAC)
  9. 5-Hydroxy-3-indoleacetic acid (5-HIAA)
  10. 4-Hydroxy-3-methoxyphenylacetic acid (HVA)

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 50 mm, 5 μm

Part Number:

186003744

Mobile Phase A:

H2O

Mobile Phase B:

ACN

Mobile Phase C:

100 mM HCOONH4, pH 3.0

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Column Temperature:

30 ˚C

Detection:

UV @ 280 nm

Instrument:

Waters Alliance 2695

Gradient

Time (min)

Profile

%A

%B

%C

0.00

95

0

5

5.00

95

0

5

15.00

70

25

5

20.00

70

25

5

21.00

95

0

5

25.00

95

0

5

Results and Discussion

1. Norepinephrine (NE), 2. Epinephrine (E), 3. 3,4-Dihydroxyphenylalanine (DOPA), 4. Dopamine (DA), 5. 3-Methoxy-4-hydroxyphenylglycol (MHPG), 6. 4-Hydroxy-3-methoxymandelic acid (VMA), 7. Serotonin(5-HT), 8. 3,4-Dihydroxyphenylacetic acid (DOPAC), 9. 5-Hydroxy-3-indoleacetic acid(5-HIAA), 10. 4-Hydroxy-3-methoxyphenylacetic acid (HVA)

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WA60207, January 2009

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