This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of propranolol in human urine using SymmetryShield columns.
Compounds used in this study are:
1. Metoprolol
2. Oxprenolol (I.S.)
3. Propranolol
            
        | 
                     Column:  | 
                     SymmetryShield RP18, 3.9 x 150 mm, 5 μm (p/n: 186000108)  | 
            
| 
                     Guard Column:  | 
                     Sentry guard column RP18 3.9 x 20 mm, 5 μm (p/n: 186000107)  | 
            
| 
                     Mobile phase:  | 
                     0.1% TFA in water/Acetonitrile 80:20  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     50 μL  | 
            
| 
                     Temperature:  | 
                     30° C  | 
            
| 
                     Detection:  | 
                     UV @ 275 nm  | 
            
            
        
            
        
            
        WA31763.134, June 2003