• Application Note

Metoprolol - pH 2.5, LC-MS

Metoprolol - pH 2.5, LC-MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of metoprolol using XTerra MS C18 columns.

Introduction

Metoprolol using LC-MS has been analyzed in this study.

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.1% HCOOH in H2O, pH 2.5

Mobile phase B:

0.1% HCOOH in CAN, pH 2.5

Isocratic mobile phase composition:

90%A; 10%B

Flow rate:

0.2 mL/min to MS

Injection volume:

20 μL of 100 pg/μL

Temperature:

Ambient

Detection:

MS ESI+, SIR 268.12

Instrument:

Alliance 2795 HT, Micromass ZQ

MS Conditions

MS sytem:

Micromass ZQ

Ion source:

ESI+

Capillary:

3.0 kV

Cone:

30 V

Extractor:

3.0

RF Lens:

0.5

Source temp.:

150 °C

Desolvation temp.:

350 °C

Cone gas flow:

60 L/hr

Desolvation gas flow:

500 L/hr

LM Resolution:

15

HM Resolution:

15

Ion energy:

1

Multiplier (V):

650

Results and Discussion

Featured Products

WA20738.072, June 2002

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