• Application Note

ACQUITY UPLC HILIC Isocratic Separation of Isoascorbic acid and ascorbic acid

ACQUITY UPLC HILIC Isocratic Separation of Isoascorbic acid and ascorbic acid
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the separation of isoascorbic acid and ascorbic acid.

Introduction

Compounds which are studied in this application brief are:

  1. Isoascorbic acid
  2. Ascorbic acid

Structures

Experimental

Test Conditions

Chromatographic Conditions

Column:

ACQUITY UPLC BEH Amide,

Isocratic Mobile Phase:

80/20 MeCN/H2O with 10 mM KH2PO4, pH 4.6

Flow Rate:

0.2 mL/min

Injection Volume:

5.0 μL (PLNO)

Sample Concentration:

30 μg/mL each

Sample Diluent:

75/25 MeCN/MeOH with 0.2% HCOOH

Column Temperature:

25 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 260nm

Sampling Rate:

20 points/sec

Filter Time Constant:

0.2

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Results and Discussion

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WA60102, June 2009

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