This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of cephalexin.
The compounds analyzed in this sudy are -
| 
                     Compounds  | 
                     USP Tailing Factors  | 
            
|---|---|
| 
                     1-hydroxybenzotriazole (IS)  | 
                     1.4  | 
            
| 
                     Cephalexin  | 
                     1.2  | 
            
            
        
            
        | 
                     Column:  | 
                     Symmetry C18, 3.0 x 150 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     WAT054200  | 
            
| 
                     Mobile phase:  | 
                     1.25% glacial acetic acid in water/methanol 88:12  | 
            
| 
                     Flow rate:  | 
                     0.6 mL/min  | 
            
| 
                     Injection volume:  | 
                     400 μL of cephalexin (1 mg/mL) and 200 μL of 1-hydroxybenzotriazole (Internal standard, 1 mg/mL)  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        WA31763.51, June 2003