• Application Note

Albuterol (Salbutamol) in Serum

Albuterol (Salbutamol) in Serum
  • Waters Corporation
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Technician holding test tube examining human centrifuged blood sample

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of Albuterol in serum using Symmetry C18 columns.

Introduction

Compounds used in this study are:

1. Salbutamol

2. Bamethan (I.S.)

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm(p/n: WAT046980)

Guard Column:

Sentry Guard Column 3.9 x 20 mm, 5 μm(p/n: WAT054250)

Mobile phase:

5 mM hexanesulfonate and 1% glacial acetic acid in water/1% glacial acetic acid in methanol, 75:25

Flow rate:

1.0 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Detection:

Fluorescence, Ex = 275 nm, Em = 310 nm

Results and Discussion

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WA31763.19, June 2003

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