• Application Note

Analysis of Antibacterials using XBridge Phenyl 

Analysis of Antibacterials using XBridge Phenyl 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of antibacterials using XBridge Phenyl columns.

Introduction

Compounds used in this study are: 1. Carbadox 2. Thiamphenicol 3. Furazolidone 4. Oxolinic Acid 5. Sulfadimethoxine 6. Sulfaquinoxaline 7. Nalidixic Acid.

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 100 mm, 3.5 μm p/n: 186003334

Mobile phase A:

20 mM KH2PO4, pH 2.5

Mobile phase B:

ACN

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample:

Carbadox (10 μg/mL), Thiamphenicol (100 μg/mL),

Furazolidone (10 μg/mL),

Oxolinic Acid (10 μg/mL),

Sulfadimethoxine (10 μg/mL),

Sulfaquinoxaline (10 μg/mL), Nalidixic Acid (10 μg/mL) in KH2PO4/ACN (80/20)

Column temp.:

30 °C

Sampling temp.:

15 °C

Detection:

UV @ 254 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

0

80

20

2

80

20

7

75

25

15

70

30

16

80

20

20

80

20

Results and Discussion

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WA60191, June 2007

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