• Application Note

Catechin Mixture: SunFire Intelligent Speed Separation

Catechin Mixture: SunFire Intelligent Speed Separation
  • Waters Corporation
Download PDF Download PDF
Dried green tea leaves

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the separation of Catechin mixture found in tea using SunFire IS Column.

Introduction

Compounds use in this study:

1. Caffeine

2. Epicatechin

3. Epigallocatechin Gallate

Experimental

Conditions

Column:

SunFire C8 4.6 x 150 mm, 5 μm

Part number:

186002549

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in Acetonitrile

Flow rate:

3 mL/min

Injection volume:

10 μL

Sample concentration:

20 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 280 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Results and Discussion

Featured Products

WA41888, March 2005

Back To Top Back To Top