• Application Note

Theophylline and Theobromine

Theophylline and Theobromine
  • Waters Corporation
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Respiratory disease

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of theophylline and theobromine using Symmetry C8 Columns.

Introduction

The compounds analyzed in this study are:

  1. Theophylline 
  2. Theobromine
  3. Caffeine 
Compounds

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm

Guard column:

Symmetry Guard Column 3.9 x 20mm, 5 μm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

10 mM potassium phosphate, pH 7.0/ methanol 76:24

Flow rate:

1.0 mL/min

Injection volume:

20 μL of 10 μg/mL sample

Detection:

UV @ 280 nm

USP Tailing Factors

  1. 1.18 
  2. 1.03 
  3. 1.01 

Results and Discussion

Results

Featured Products

WA31763.170, June 2003

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