• Application Note

Omeprazole in Rat Plasma

Omeprazole in Rat Plasma
  • Waters Corporation
Download PDF Download PDF
Plasma in the hands of a nurse in surgical operation room

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of omeprazole in rat plasma using Oasis MCX extraction method.

Introduction

Omeprazole in rat plasma is being analyzed in this application brief.

Experimental

HPLC Method

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

1.0% NH4OH

Mobile phase B:

ACN

Isocratic mobile phase composition:

50% A; 50% B

Flow rate:

0.2 mL/min

Injection volume:

20 μL

Detection:

MS ESI+

Instrument:

Alliance 2690, Quattro Ultima

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 10 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

30 V

Oasis MCX Extraction Method

Oasis MCX Extraction Plate, 10 mg/96-well Part Number 186000259

 

Results and Discussion

Featured Products

WA31764.111, June 2003

Back To Top Back To Top