This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of flavanoids.
The compounds used in this study are –
|
Column: |
Xterra Phenyl, 4.6 x 150 mm, 5 μm |
|
Part number: |
186001146 |
|
Mobile phase A: |
H2O |
|
Mobile phase B: |
MeOH |
|
Mobile phase C: |
0.1% H3PO4, pH 3.0 |
|
Flow rate: |
1.0 mL/min |
|
Isocratic mobile phase composition: |
48% A; 42% B; 10% C |
|
Injection volume: |
20 μL |
|
Temperature: |
35 °C |
|
Detection: |
UV @ 270 nm |
|
Instrument: |
Alliance 2695, 2996 PDA |
WA20738.052, June 2002