This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of ephedra alkaloids.
The compounds used in this study are –
            
        | 
                     Column:  | 
                     Xterra Phenyl, 2.1 x 150 mm, 3.5 μm  | 
            
| 
                     Part number:  | 
                     186001181  | 
            
| 
                     Mobile phase A:  | 
                     10 mM NH4HCO3, pH 9.5  | 
            
| 
                     Mobile phase B:  | 
                     ACN  | 
            
| 
                     Flow rate:  | 
                     0.23 mL/min  | 
            
| 
                     Isocratic mobile phase composition:  | 
                     90% A; 10% B  | 
            
| 
                     Injection volume:  | 
                     5 μL  | 
            
| 
                     Temperature:  | 
                     40 °C  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2695, 2996 PDA  | 
            
            
        WA20738.048, June 2002