 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of cephalexin.
The compounds analyzed in this sudy are -
| Compounds | USP Tailing Factors | 
|---|---|
| 1-hydroxybenzotriazole (IS) | 1.4 | 
| Cephalexin | 1.2 | 
 
            
         
            
        | Column: | Symmetry C18, 3.0 x 150 mm, 5 μm | 
| Part number: | WAT054200 | 
| Mobile phase: | 1.25% glacial acetic acid in water/methanol 88:12 | 
| Flow rate: | 0.6 mL/min | 
| Injection volume: | 400 μL of cephalexin (1 mg/mL) and 200 μL of 1-hydroxybenzotriazole (Internal standard, 1 mg/mL) | 
| Detection: | UV @ 254 nm | 
 
            
        WA31763.51, June 2003