This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of anilines.
The compounds used in this study are –
            
        | 
                     Column:  | 
                     Xterra MS C18 4.6 x 20 mm, 3.5 μm  | 
            
| 
                     Part number:  | 
                     186001891  | 
            
| 
                     Mobile phase A:  | 
                     H2O  | 
            
| 
                     Mobile phase B:  | 
                     ACN  | 
            
| 
                     Mobile phase C:  | 
                     100 mM NH4HCO3, pH 10.0  | 
            
| 
                     Flow rate:  | 
                     3.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     5.0 μL  | 
            
| 
                     Sample concentration:  | 
                     20 μg/mL  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
| 
                     Instrument:  | 
                     Alliance HT 2795, 2996 PDA  | 
            
            
        
            
        WA20738.011, June 2002