• Application Note

Analysis of Statins using XBridge Phenyl 

Analysis of Statins using XBridge Phenyl 
  • Waters Corporation
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Statins, beta blockers, or generic tablets

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of statins using XBridge Phenyl columns.

Introduction

Compounds

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 100 mm, 3.5 μm p/n: 186003334

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3

Flow rate:

1.2 mL/min

Injection volume:

20 μL

Sample:

Atorvastatin (10 μg/mL),

Simvastatin (10 μg/mL),

Lovastatin (10 μg/mL),

Pravastatin Sodium (10 μg/mL), in H2O/NH4HCO3 (90/10)

Column temp.:

25 °C

Sample temp.:

15 °C

Detection:

UV @ 248 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time (min)

%A

%B

%C

0

65

15

20

20

30

50

20

21

65

15

20

25

65

15

20

Results and Discussion

chromatogram
Compounds: 1. Pravastatin Sodium 2. Lovastatin 3. Simvastatin 4. Atorvastatin. 

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