Analysis of Non-Steroidal Inflammatory Drugs (NSAIDS) using XBridge Phenyl

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of non-steroidal inflammatory drugs using XBridge Phenyl columns.

Columns:	XBridge Phenyl, 4.6 x 100 mm, 3.5 μm p/n: 186003334
Mobile phase A:	H₂O
Mobile phase B:	MeOH
Mobile phase C:	0.2% HCOOH in H₂O
Flow rate:	1.0 mL/min
Injection volume:	15 μL
Sample:	Fenoprofen (50 μg/mL), Ibuprofen (20 μg/mL), Diclofenac (10 μg/mL), Tolmetin (10 μg/mL), Suprofen (10 μg/mL), Naproxen (10 μg/mL) in H₂O/MeOH (60/40)
Column temp.:	30 °C
Sampling temp.:	15 °C
Detection:	UV @ 254 nm
Sampling Rate:	5 points/sec
Filter Response:	0.2
Instrument:	Alliance 2695 with 2996 PDA

Time(min)	%A	%B	%C
13.33	25	65	10
16.67	25	65	10
17.33	40	50	10
20.00	40	50	10

Compounds: 1. Suprofen 2. Tolmetin 3. Naproxen 4. Fenoprofen 5. Ibuprofen 6. Diclofenac.