 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of steroids in formic acid.
The compounds used in this study are -
 
            
        | Column: | SunFire C18 4.6 x 150 mm, 5 μm | |
| Part number: | 186002559 | |
| Mobile phase A: | 0.1% HCOOH in Water | |
| Mobile phase B: | 0.1% HCOOH in ACN | |
| Flow rate: | 1.4 mL/min | |
| Injection volume: | 10 μL | |
| Sample concentration: | 50 mg/mL each, in DMSO | |
| Temperature: | Ambient | |
| Detection: | UV @ 254 nm | |
| Instrument: | AutoPurification System | 
 
            
         
            
        WA41899, March 2005