• Application Note

Catechins, pH 10.0 – 4.6 x 20 mm Intelligent Speed Separation

Catechins, pH 10.0 – 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of catechins using Symmetry Columns. 

Introduction

Structures

Structures

Experimental

Conditions

Column:

Symmetry C18, 4.6 x 20 mm IS, 3.5 μm, (P/N: 186002090) SymmetryShield RP18, 4.6 x 20 mm IS, 3.5 μm, (P/N: 186002092)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in Methanol

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Sample concentration:

20 μg/mL

Temperature:

30°C

Detection:

UV @ 280 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Gradient

Results and Discussion

The compounds analyzed in this study are:

  1. Caffeine
  2. Epicatechin
  3. Epigallocatechin Gallate 

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WA31787.12, June 2003

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