BioResolve Protein A Affinity Columns
An affinity for sensitivity, productivity, and simplicity
Accelerate antibody titer measurements with Waters BioResolve Protein A Affinity Columns featuring MaxPeak Premier Technology. These high-performance columns help you achieve up to 7x greater sensitivity earlier in cell harvest, enabling faster decision-making and better process control. Obtain titer information earlier to build confidence in clone selection, mitigate sample and batch loss, and decrease time to downstream processes and quality control.
With the simplified Direct Connect 2D method, you can streamline your workflows by allowing seamless integration with SEC using a single-pump UHPLC system. Take advantage of the ability to collect 2x more information per run while removing unnecessary complications. Achieve reliable, reproducible results for titer and aggregation quantification with Waters BioResolve Protein A Affinity Columns.
BioResolve Protein A Affinity Columns
Specifications
Overview
- Improve peak shape and sensitivity for monoclonal antibodies (mAbs), multi-specifics, and conjugates
- Obtain reproducible results injection-to-injection and batch-to-batch
- Access valuable antibody titer information earlier in cell harvest to prevent batch loss
- Ensure compatibility with multiple LC systems due to high-pressure tolerance
- Balance robustness, flexibility, and sensitivity with the 3.9 mm column, compatible with any HPLC, UHPLC, or UPLC for ultimate versatility
- Maximize sensitivity and achieve the sharpest peaks possible using the 2.1 mm column with UHPLC or UPLC
- Simplify 2D ProA-SEC using a single-pump UHPLC system with an autosampler and UV detector, with ProA and SEC columns connected in series
Recommended Use: For analytical development, process development, and PAT labs seeking optimal sensitivity and reproducibility for cell harvesting.
BioResolve Protein A Affinity Columns Technologies and Characteristics
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Reduce analyte/surface interactions -
Increased analyte recovery
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Better peak shape
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Increase sensitivity
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Non-porous (strong and fast)
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3.5 µm with mechanical strength for maximum column efficiency (high efficiency)
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Hydrophilic coating added (low secondary interactions) to counteract hydrophobic nature of DVB-PS particle
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Improved peak shape
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Enhanced peak shape for easier analysis
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Greater sensitivity for mAbs, multi-specifics, and conjugates
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Faster decision-making and better process control
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Reliable reproducibility injection-to-injection and batch-to-batch
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Simplified 2D applications (e.g., ProA-SEC) on all LC systems including UPLC
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High-pressure tolerance for efficient quality attribute assessment
Highest sensitivity titer
Achieve improved peak shape using a novel affinity particle and MaxPeak Premier Technology. To get even more out of every run, employ a simplified, direct-connect workflow using a single-pump UHPLC system for an easier, more reliable 2D ProA-SEC setup than traditional switching-valve, dual-pump, and loop-based methods. Gain greater titer sensitivity and improved lower limits of quantitation (LLOQ) for mAbs, multi-specifics, and conjugates. Enable earlier access to results in upstream processing and drive faster method optimization in downstream development to support critical applications.
Improved productivity through reproducibility
Achieve a high degree of injection-to-injection reproducibility, with less than 1% relative standard deviation (%RSD) for area across 30 titer injections. The example shown uses a 2.1 x 20 mm column with 1 µg injections at 1.0 mL/min.
Simplified 2D technology
Experience an uncomplicated 2D ProA-SEC setup for increased reliability compared to traditional 2D methods using switching valves, dual pumps, and holding loops. Achieve reproducible titer and comparative high molecular weight species (HMWS) abundances for mAbs, with the capability to track and potentially quantify mAb low molecular weight species (LMWS) that exhibit Protein A affinity binding.
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