This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights analysis of nucleic acid bases
|
Column: |
Atlantis T3, 4.6 x 150 mm, 5 m |
|
Part Number: |
186003747 |
|
Mobile Phase A: |
H2O |
|
Mobile Phase B: |
ACN |
|
Mobile Phase C: |
100 mM CH3COONH4, pH 5.0 |
|
Flow Rate: |
1.0 mL/min |
|
Injection Volume: |
10 μL |
|
Column Temperature: |
30˚C |
|
Detection: |
UV @ 254 nm |
|
Instrument: |
Waters Alliance 2695 |
|
Time(min) |
Profile |
||
|---|---|---|---|
|
%A |
%B |
%C |
|
|
0 |
90 |
0 |
10 |
|
10 |
84 |
6 |
10 |
|
11 |
90 |
0 |
10 |
|
15 |
90 |
0 |
10 |
1. Cytosine, 2. 5-Fluorocytosine, 3. Uracil, 4. 5-Fluorouracil, 5. Guanine, 6. Thymine, 7. Adenine.
WA60208, January 2009
