• Application Note

Tricyclic Antidepressants Using LC-MS

Tricyclic Antidepressants Using LC-MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of tricyclic antidepressants using LC-MS.

Introduction

The compounds used in this study are -

  1. Trimipramine
  2. Triprolidine
  3. Doxepin
  4. Desipramine
  5. Amitriptyline

Experimental

HPLC Conditions

Column:

SunFire C8 2.1 x 20 mm IS, 3.5 μm

Part number:

186002697

Mobile phase A:

Water with 0.1% HCOOH

Mobile phase B:

Acetonitrile with 0.1% HCOOH

Flow rate:

0.63 mL/min

Injection volume:

2 μL

Sample concentration:

20 μg/mL in water

Temperature:

30 °C

Detection:

Waters Micromass ZQ

Instrument:

Alliance 2796 with 2487 Dual Wavelength Detector

MS Conditions

ESI+ capillary (kV):

3

Cone voltage (V):

30

Extractor (V):

3

RF lens (V):

0.3

Source temp. (°C):

150

Desolvation temp. (°C):

350

Cone gas flow (L/hr):

50

Desolvation flow (L/hr):

550

Results and Discussion

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