This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of antidepressants in TFA.
The compounds used in this study are -
SunFire Column mass loading comparison for basic compounds (tricyclic antidepressants) in TFA. Comparison made with other leading HPLC column brands using 3,000 μg total load.
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                     Column:  | 
                     SunFire C18 4.6 x 150 mm, 5 μm  | 
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                     Part number:  | 
                     186002559  | 
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                     Mobile phase A:  | 
                     0.1% TFA in Water  | 
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                     Mobile phase B:  | 
                     0.1% TFA in ACN  | 
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| 
                     Flow rate:  | 
                     1.4 mL/min  | 
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                     Injection volume:  | 
                     10 μL  | 
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                     Sample concentration:  | 
                     100 mg/mL each, in DMSO  | 
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                     Temperature:  | 
                     Ambient  | 
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                     Detection:  | 
                     UV @ 254 nm  | 
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                     Instrument:  | 
                     AutoPurification System  | 
            
WA41905, May 2005