 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of clobetasol using SymmetryShield RP18 columns.
Clobetasol is analyzed in this application brief.
 
            
        | Column: | SymmetryShield RP18, 4.6 x 150 mm, 5 μm (p/n: 186000109) | 
| Mobile phase: | Acetonitrile/water/methanol 48:42:10 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 10 μL | 
| Detection: | UV @ 240 nm | 
1. 1.07
2. 1.05
 
            
        WA31763.59, June 2003