• Application Note

Ampicillin

Ampicillin
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of Ampicillin using Symmetry C8 Columns. 

Introduction

The compound analyzed in this study is Ampicillin. 

Compounds

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 µm

Guard column:

Symmetry Guard Column 3.9 x 20 mm, 5 µm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

10 mM sodium acetate, pH 4.0/acetonitrile 92:8

Flow rate:

1.0 mL/min

Injection volume:

10 µL of 100 µg/mL sample

Detection:

UV @ 230 nm

USP Tailing Factors

  1. 1.07 

Results and Discussion

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WA31763.25, June 2003

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