Gradient Separation of Histidine Dipeptides on ACQUITY UPLC BEH HILIC

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 50 mm, 1.7 μm

Part Number:

186003460

Mobile Phase A:

50/50 ACN/10 mM ammonium formate, w/ 0.125% HCOOH, pH 3.0

Mobile Phase B:

95/5 ACN/10 mM ammonium formate, w/ 0.125% HCOOH, pH 3.0

Flow Rate:

0.5 mL/min

Injection Volume:

5.0 μL

Sample Diluent:

75:25 ACN:MeOH

Sample Concentration:

Creatinine 1μg/mL; Carnosine 5 μg/mL; Anserine 5 μg/mL; Creatine 5 μg/mL

Column Temperature:

30 °C

Weak Needle Wash:

ACN/H₂O 95/5

Instrument:

Waters ACQUITY UPLC with ACQUITY SQD

Ionization Mode:

ES+

Capillary:

2.5 kV

Cone:

20 V (Carnosine; Creatinine, Anserine);

25 V (Creatine)

Source Temperature:

120 °C

Desolvation Temperature:

400 °C

Desolvation Gas Flow:

800 L/Hr

Cone gas Flow:

5 L/Hr

SIR m/z:

227.1 m/z (Carnosine); 132.1 m/z (Creatine); 114.05 m/z (Creatinine); 241.1 m/z (Anserine)

Dwell Time:

0.1 s