• Application Note

Analysis of Catechins Using Atlantis T3 Columns

Analysis of Catechins Using Atlantis T3 Columns
  • Waters Corporation
Download PDF Download PDF
Hand picking fresh tea leaves in natural organic green tea farm

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of catechins using Atlantis T3 Columns.

Introduction

The compounds used in this study are - 

  1. Gallic acid
  2. Epigallocatechin
  3. Catechin
  4. Caffeine
  5. Epigallocatechin gallate
  6. Epicatechin
  7. Epicatechin gallate
  8. Catechin gallate

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 μm

Part number:

186003747

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

1% HCOOH in H2O, pH 2.3

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample:

Gallic Acid (10 μg/mL),

Epigallocatechin (40 μg/mL),

Catechin (20 μg/mL),

Caffeine (10 μg/mL),

Epigallocatechin Gallate (20 μg/mL),

Epicatechin (40 μg/mL),

Epicatechin Gallate (20 μg/mL),

Catechin Gallate (20 μg/mL),

in H2O/MeOH (85/15)

Column temperature:

25 °C

Sample temperature:

15 °C

Detection:

UV @ 280 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time (min)

Profile

%A

%B

%C

0.00

75

20

5

10.00

65

30

5

20.00

35

60

5

21.00

75

20

5

25.00

75

25

5

Results and Discussion

Featured Products

WA60224, January 2009

Back To Top Back To Top