• Application Note

Steroids in Serum

Steroids in Serum
  • Waters Corporation
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Prednisone pills and bottle pour out on table

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids in serum.

Introduction

The compounds used in this study are – 

  1. Prednisolone
  2. Betamethasone
  3. Butyl Paraben (I.S.)
  4. Betamethasone Valerate

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.9 x 150 mm, 5 μm

Guard column:

Sentry Guard Column 3.9 x 20 mm, 5μm

Part numbers:

Column - WAT200655, Guard - WAT200675

Mobile phase:

20 mM potassium phosphate, pH 7/methanol 41:59

Flow rate:

1.0 mL/min

Injection volume:

5 μL of reconstituted porcine serum extract

Detection:

UV @ 254 nm

Results and Discussion

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample.

Featured Products

WA31763.150, June 2003

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