• Application Note

Pharmaceutical Test Mix - 4.6 x 20 mm Intelligent Speed Separation

Pharmaceutical Test Mix - 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of pharmaceutical test mix using Symmetry and SymmetryShield RP18 columns.

Introduction

Compounds used in this application brief are:

1. 8-Bromoguanosine

2. Acetanilide

3. Triamcinolone

4. Hydrocortisone

5. 2-Amino-7-chloro-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carbonitrile

6. 6λMethyl-17λ-hydroxyprogesterone

7. 3-Aminofluoranthene

8. 2-Bromofluorene

9. Perylene

10. Naphtho(2,3-a)pyrene

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 20 mm, 3.5 μm IS (p/n: 186002090)

SymmetryShield RP18, 4.6 x 20 mm, 3.5 μm IS (p/n: 186002092)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in ACN

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Temperature:

30° C

Detection:

UV @ 254 nm

Gradient

Results and Discussion

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WA31763.123, June 2003

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