• Application Note

Oxybutynin in Rat Plasma - Xterra MS C18 Column

Oxybutynin in Rat Plasma - Xterra MS C18 Column
  • Waters Corporation
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Plasma preparation for centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of oxybutynin in rat plasma.

Introduction

The compound analyzed in this study is oxybutynin.

Experimental

HPLC Method

Column:

Xterra MS C18 2.1 x 30 mm, 3.5 μm

Part number:

186000398

Mobile phase A:

100 mM NH4COOH

Mobile phase B:

ACN

Isocratic mobile phase composition:

45% A; 55% B

Flow rate:

0.2 mL/min

Injection volume:

30 μL

Detection:

MS ESI+

Instrument:

Alliance 2790, Quattro Ultima

Ion source:

Electrospray Positive

Source temperature:

150 °C

Gas cell:

1.5e-3 mbar, 25eV

Desolvation temperature:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

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WA31764.115, June 2003

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