This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights on the desalting of DNA oligonucleotides by SPE for MALDI analysis.
DNA oligonucleotides are retained on the Oasis HLB sorbent by ion-pair reversed-phase mechanism. A volatile ion-pair agent is used as loading buffer* (0.1 triethylamine acetate, pH ~ 7).
The Oasis HLB µElution 96 well plate has sufficient capacity for desalting 1 pmol up to 5,000 pmol of oligonucleotides sample.
WA31764.65, June 2003