This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the gradient separation of morphine and its metabolites on Atlantis HILIC Silica column.
The compounds used in this study are-
Figure 1. Structures of the compounds used in this study.
|
Column: |
Atlantis HILIC Silica, 2.1 x 50 mm, 3 μm |
|
Part Number: |
186002011 |
|
Mobile Phase A: |
10 mM NH4COOH in H2O, 0.125% HCOOH in 50:50 ACN:H2O |
|
Mobile Phase B: |
10 mM NH4COOH in H2O, 0.125% HCOOH in 90:10 ACN:H2O |
|
Flow Rate: |
0.6 mL/min |
|
Injection Volume: |
5 μL |
|
Sample Concentration: |
25 μg/mL each |
|
Sample Diluent: |
75:25 ACN:MeOH with 0.2% HCOOH |
|
Column Temperature: |
30 °C |
|
Detection: |
UV @ 280 nm |
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Sampling Rate: |
20 points/sec |
|
Time Constant: |
0.1 |
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Instrument: |
Waters ACQUITY UPLC with ACQUITY PDA |
|
Time(min) |
Profile |
|
|---|---|---|
|
%A |
%B |
|
|
0.00 |
0.1 |
99.9 |
|
1.05 |
0.1 |
99.9 |
|
4.35 |
99.9 |
0.1 |
|
4.50 |
0.1 |
99.9 |
|
6.00 |
0.1 |
99.9 |
WA64070, August 2009
