• Application Note

Gradient Separation of Catecholamines on Atlantis HILIC Silica

Gradient Separation of Catecholamines on Atlantis HILIC Silica

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief describes the gradient separation of catecholamines on Atlantis HILIC Silica column.

Introduction

The compounds used in this study are:

  1. Norepinephrine (NE)
  2. Epinephrine (E)
  3. 3-Hydroxytyramine (Dopamine)

Experimental

Test Conditions

Column:

Atlantis HILIC Silica, 4.6 x 50 mm, 5 μm

Part Number:

186002028

Mobile Phase A:

H2O

Mobile Phase B:

ACN

Mobile Phase C:

200 mM NH4COOH, pH 3.0

Flow Rate:

2.0 mL/min

Injection Volume:

5.0 μL

Sample Diluent:

75:25 ACN:MeOH with 0.2% FA

Sample Concentration:

42 μg/mL Norepinephrine (NE), 17 μg/mL Epinephrine (E), 83 μg/mL 3-hydroxytyramine (Dopamine)

Detection:

UV @ 280 nm

Temperature:

Ambient

Instrument:

Waters Alliance System, 2695 Separations Module with 2996 PDA

Gradient:

Time(min)

Profile

%A

%B

%C

0.0

0

95

5

5.0

25

7

5

Results and Discussion

Featured Products

WA64073, August 2009

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