This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of nimodipine using XBridge Shield RP18 columns.
            
        | 
                     Columns:  | 
                     XBridge Shield RP18 4.6 x 100 mm, 3.5 μm p/n: 1860003044  | 
            
| 
                     Mobile phase A:  | 
                     200 mM Ammonium formate pH 3  | 
            
| 
                     Mobile phase B:  | 
                     acetonitrile  | 
            
| 
                     Mobile phase C:  | 
                     H2O  | 
            
| 
                     Flow rate:  | 
                     1.4 mL/min  | 
            
| 
                     Injection:  | 
                     100 μL @ 30 mg/mL in DMSO  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2695 with 2996 PDA  | 
            
| 
                     Time (min)  | 
                     %A  | 
                     %B  | 
                     %C  | 
            
|---|---|---|---|
| 
                     0  | 
                     10  | 
                     30  | 
                     60  | 
            
| 
                     50  | 
                     10  | 
                     60  | 
                     30  | 
            
            
        WA60171, April 2007