This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on the analysis of nalidixic acid antibiotics by LC-MS.
Column: |
Atlantis dC18, 2.1 x 20 mm IS, 3.0 μm, (P/N: 186002058) |
Mobile phase A: |
Water |
Mobile phase B: |
Methanol |
Mobile phase C: |
1% HCOOH in Water |
Flow rate: |
0.4 mL/min |
Injection volume: |
2 μL |
Sample concentration: |
10 μg/mL |
Temperature: |
30°C |
Instrument: |
Alliance 2795 and Waters ZQ |
Waters ZQ |
|
ES+ |
|
Capillary (kV): |
3.5 |
Cone (V): |
5.0 |
Extractor: |
3.0 |
RF lens: |
0.1 |
Source temp. (°C): |
150 |
Desolvation temp. (°C): |
400 |
Cone gas flow (L/Hr): |
50 |
Desolvation gas fLow(L/Hr): |
500 |
LM resolution: |
15 |
HM resolution: |
15 |
Ion energy: |
0.5 |
Multiplier (V): |
650 |
The top figure is the total ion current, followed by the extracted ion signals for each of the three analytes.
*The “extra” peak in the cinoxacin panel is the isotope from oxolinic acid.
WA31787.16, June 2003