This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the analysis of Lidocaine HCL using Symmetry Columns.
The compound analyzed in this study is Lidocaine HCL.
            
        | 
                     Column:  | 
                     Symmetry Shield RP18, 4.6 x 250 mm, 5 µm  | 
            
| 
                     Part number:  | 
                     186000112  | 
            
| 
                     Mobile phase:  | 
                     50 mL glacial acetic acid in 930 mL of water, pH adjusted to 3.4 with 0.1 N sodium hydroxide/70% buffer/30% acetonitrile  | 
            
| 
                     Flow rate:  | 
                     1.5 mL/min  | 
            
| 
                     Injection volume:  | 
                     20 µL  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        WA31763.95, June 2003