This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of flavonoids in Ginkgo
The compounds analyzed in this application brief are quercetin, kaempferol, and isorhamnetin
1 g sample is refluxed in 50 mL of ethanol: 3M HCl (70:30) for 2.5 hr. The cooled sample is adjusted to exactly 100 mL 0.3 mL of the ethanolic extract is diluted 1:10 with water.
Column: |
Symmetry C18, 4.6 x 250 mm, 5 μm |
Part number: |
WAT054275 |
Mobile phase: |
0.5% Phosphoric acid/Methanol 50:50 |
Flow rate: |
1.5 mL/min |
Injection volume: |
10 μL |
Temperature: |
25 °C |
Detection: |
UV @ 270nm (0.02 AUFS) |
WA31764.83, June 2003