• Application Note

Ecstacy (MDMA) and Metabolites by LC-MS/MS

Ecstacy (MDMA) and Metabolites by LC-MS/MS
  • Waters Corporation
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Application Note

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights about the analysis of MDMA and its metabolites by LC-MS/MS method

Introduction

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 150 mm, 3.5 µm

Part number:

186000408

Mobile phase A:

20 mM NH4HCO3, pH 9.0

Mobile phase B:

MeOH

Flow rate:

0.2 mL/min

Isocratic mobile phase composition:

70% A; 30% B

Injection volume:

15 µL

Temperature:

30 °C

Detection:

MS ESI+

Instrument:

Alliance 2695, Micromass ZQ

MS Conditions

Instrument:

Waters/Micromass ZQ

Ion source:

Electrospray Positive

Source temperature:

150˚C

Desolvation temperature:

350˚C

Results and Discussion

Ions Monitored

Ions Monitored

Compounds

  1. 4-Hydroxy-3-methoxymetmamphetamine (HMMA). 
  2. 3,4-Methylenedioxyamphetamine (MDA) 
  3. MDMA-D5 (ISTD) 
  4. 3,4-Methylenedioxymethamphetamine (MDMA)

Analysis at pH 9 allows good peak shape and maximum retention for basic compounds  with no modifiers that can interfere with LC-MS analysis. Do not try this with traditional  silica based columns.

Featured Products

WA31764.72, June 2003

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