This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on the analysis of antipyrine and its metabolites using Symmetry Columns.
The compounds analyzed in this study are:
 
            
        | Column: | Symmetry Shield RP8, 4.6 x 50 mm, 5µm | 
| Part number: | 186000224 | 
| Mobile phase: | 0.1% glacial acetic acid/methanol 85:15 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 5 µL solution with antipyrine (240 ng) and 4-hydroxyantipyrine (29 ng) | 
| Detection: | UV @ 254 nm | 
 
            
        WA31763.31, June 2003