This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of doxepin.
The compound analyzed in this study is doxepin.
            
        | 
                     Column:  | 
                     Xterra RP18 4.6 x 150 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     186000492  | 
            
| 
                     Mobile phase:  | 
                     pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 40:50:10 pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 50:40:10 pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 20:70:10  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     5 μL of 250 μg/mL  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 260 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2695, 2996 PDA  | 
            
| 
                     Mobile Phase pH  | 
                     USP Tailing  | 
            
|---|---|
| 
                     3.0  | 
                     1.25  | 
            
| 
                     7.0  | 
                     1.36  | 
            
| 
                     10.0  | 
                     1.17  | 
            
            
        WA20738.044, June 2002