• Nota de aplicación

Gradient Separation of Nutrients on Atlantis HILIC Silica

Gradient Separation of Nutrients on Atlantis HILIC Silica
  • Waters Corporation
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Stack of vitamins

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the gradient separation of nutrients on Atlantis HILIC Silica Columns.

Introduction

The componds used in this study are:

  1. Nicotinamide
  2. Nicotinic acid
  3. Thiamine

Experimental

Method Conditions

Column:

Atlantis HILIC Silica, 2.1 x 50 mm, 3 μm

Part Number:

186002011

Mobile Phase A:

10 mM NH4COOH in H2O, 0.125% HCOOH in 50:50 ACN:H2O

Mobile Phase B:

10 mM NH4COOH in H2O, 0.125% HCOOH in 90:10 ACN:H2O

Flow Rate:

0.6 mL/min

Injection Volume:

5 μL

Sample Concentration:

25 μg/mL each

Sample Diluent:

75:25 ACN:MeOH with 0.2% HCOOH

Column Temperature:

30 °C

Detection:

UV @ 268 nm

Sampling Rate:

40 points/sec

Filter Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY PDA

Gradient:

Time(min)

Profile

%A

%B

0.00

0.1

99.9

1.05

0.1

99.9

4.35

99.9

0.1

4.50

0.1

99.9

6.00

0.1

99.9

Results and Discussion

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WA64072, August 2009

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