• Nota de aplicación

Gradient Analysis of Acyclovir Using Atlantis T3 Columns

Gradient Analysis of Acyclovir Using Atlantis T3 Columns
  • Waters Corporation
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Acyclovir is a guanosine analogue antiviral medication

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates gradient analysis of acyclovir using Atlantis T3 Columns.

Introduction

The compound used in this study is Acyclovir.

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 50 mm, 3 μm

Part number:

186003726

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM HCOONH4, pH 3.0

Flow rate:

1.0 mL/min

Injection volume:

5 μL

Sample:

Acyclovir (10 μg/mL) in H2O

Column temperature:

30 ˚C

Sample temperature:

20 ˚C

Detection:

UV @ 254 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time (min)

Profile

%A

%B

%C

0.00

90

0

10

5.00

85

0

10

6.00

90

0

10

10.00

90

0

10

Results and Discussion

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WA60214, January 2009

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