• Nota de aplicación

Tricyclic Antidepressants

Tricyclic Antidepressants
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of tricyclic antidepressants.

Introduction

The compounds used in this study are - 

Experimental

Conditions

Column:

SunFire C18 4.6 x 150 mm, 5 μm

Part number:

186002559

Mobile phase A:

Water

Mobile phase B:

MeOH

Mobile phase C:

100 mM CH3COO-NH4+, pH 6.0

Flow rate:

1 mL/min

Isocratic mobile phase conditions:

18% A, 72% B, 10% C

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

Excellent peak shape on basic tricyclic antidepressants with SunFire C18 analytical Column.

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WA41907, May 2005

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