• Nota de aplicación

SunFire C8: A Unique RPLC Stationary Phase for Best Peak Shapes 

SunFire C8: A Unique RPLC Stationary Phase for Best Peak Shapes 
  • Fang Xia
  • Kim Van Tran
  • Diana Swanson
  • Pamela C. Iraneta
  • Diane M. Diehl
  • Waters Corporation
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Variety of medicines and drugs

Abstract

This application note highlights about the benefits of SunFire C8 Columns.

Benefits

The new SunFire C8 Column is a reversedphased (RP) HPLC column designed for the best peak shape, excellent efficiency, highpreparative mass loading, and superior stability under low-pH conditions. 

Introduction

HPLC columns with alkyl chains (e.g. C18 and C8) are the most popular columns used in a variety of industries. For a given RPLC substrate with a given bonding technology, the retention increases with increasing alkyl chain length due to higher hydrophobic interactions. The advantages of using shorter chain length ligands include decreased total run time, improved peak tailing, and predictable selectivity for easy methodology transfer. The SunFire C8 Columns are engineered with highly pure raw materials and a tightly controlled synthesis process. This column provides high efficiencies and symmetric peak shapes for the analysis of acids, neutrals, and bases. The columns also exhibit superior lifetimes under low-pH conditions. 

Experimental

Experimental Conditions (Figure 1)

Columns:

SunFire C8 4.6 x 150 mm, 5 µm and SunFire C18 4.6 x 150 mm, 5 µm

Flow rate:

1.0 mL/min

Isocratic:

55:35:10 (water: acetonitrile: 2% acetic acid)

Injection volume:

10 µL

Sample:

Oxazepam, lorazepam, desmethyldiazepam, temazepam, and diazepam at concentration of 10 µg/mL each in water

Detection:

UV at 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Experimental Conditions (Figure 2)

Columns:

SunFire C8, Luna C8 (2), Ace 5 C8, all columns are 3.0 x 50 mm, 5 µm

Mobile phase:

1% TFA in water

Injection volume:

5 µL

Sample:

Ethylparaben at concentration of 0.64 mg/mL

Results and Discussion

The separation of five central nervous system (CNS) depressants on the analytical SunFire C8 and SunFire C18 Columns are shown in Figure 1. As noted, total run time has decreased from 24 min on a C18 column to 16 min on the C8 Column. Furthermore, better peak shapes were observed on the C8 Column.

Figure 2 is the SunFire C8 Column lifetime study under accelerated low-pH stability conditions. SunFire C8 columns have improved low-pH stability compared to leading silica-based C8 Columns due to the unique bonding and end-capping technology. 

Conclusion

Faster separations, improved peak shape, and superior stability under low-pH conditions are observed with SunFire C8 Columns. 

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WA41940, June 2005

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