• Nota de aplicación

Propranolol in Rat Plasma by Mixed-Mode SPE and LC-MS/MS (Low Elution Volume)

Propranolol in Rat Plasma by Mixed-Mode SPE and LC-MS/MS (Low Elution Volume)
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of Propanolol in Rat Plasma by LC-MS/MS. 

Introduction

The compound analyzed in this study is Propanolol. 

Experimental

HPLC Method

Column:

XTerra MS C18, 2.1 x 30 mm, 3.5 µm

Part number:

186000398

Mobile phase A:

Water + 0.5 % NH4OH

Mobile phase B:

ACN + 0.5 % NH4OH

Flow rate:

0.2 mL/min

Temperature:

Ambient

LC instrument:

Alliance 2795

Gradient

Time (min)

Profile

%A

%B

0

5

95

1

95

5

MS Conditions

MS instrument:

Micromass Quattro Triple Quadrupole

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

2.0 e-3 bar Argon

Desolvation temp.:

350 ˚C

Drying gas flow:

500 L/hr

Cone gas flow:

50 L/hr

Cone voltage:

25 V

Collision energy:

20

Capillary voltage:

3.5 Kv

MRM transition:

Metoclopramide (IS) m/z 299.8 → 226.7

Propranolol m/z 259.9 →154.9

Amitriptyline m/z 278.1 →232.9

Nortriptyline m/z 263.9 →190.8

Results and Discussion

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WA31764.132, June 2003

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