• Nota de aplicación

Oxycodone in Serum

Oxycodone in Serum
  • Waters Corporation
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Plasma serum preparation in syringe

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of oxycodone in serum using SymmetryShield RP8.

Introduction

Compounds used in this application brief are Oxycodone and Naltrexone (I.S.).

Experimental

HPLC Method

Column:

SymmetryShield RP8, 3 x 150 mm, 5 μm (p/n: WAT094243)

Guard column:

Sentry RP8, guard column, 3.9 x 20 mm, 5 μm (p/n: WAT200675)

Mobile phase:

20 mM ammonium acetate, pH 5/acetonitrile, 90:10

Flow rate:

0.6 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Temperature:

25° C

Detection:

UV @240 nm

Results and Discussion

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WA31763.114, June 2003

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