• Nota de aplicación

Chlorzoxazone in Rat Plasma Using XTerra Columns

Chlorzoxazone in Rat Plasma Using XTerra Columns
  • Waters Corporation
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Doctor with mask and gloves holding test tube with separated plasma

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of chlorzoxazone using XTerra MS C18 columns.

Introduction

Chlorzoxazone is analyzed in rat plasma in this application brief.

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.2% HCOOH

Mobile phase B:

ACN

Flow rate:

0.2 mL/min

Isocratic mobile phase composition:

45% A; 55% B

Injection volume:

20 μL

Detection:

MS ESI-

Instrument:

Alliance 2790, Micromass Quattro Ultima

MS Conditions

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 20 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying ga flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

Featured Products

WA20738.025, June 2002

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